Amicon ultra-15 centrifugal filter units manual

Hi! I need to centrifuge using amicon ultra to concentrate my protein. I have some doubts. First of all, if I can do it at 4 ºC, if it would be better for proteins, although in the protocol it. " Amicon® Ultra Centrifugal Filter Units are useful for protein purification and concentration. This filter device can remove proteins bellow 30 kDa. This filter device can be used to concentrate a pure protein with molecular mass above 30 kDa. The recommended velocity to spin this device is RPM, however when the filter is used for. Amicon® Ultra-4 and Centrifugal Filter Units – 10, NMWL – For in vitro diagnostic use (CE) Centrifugal filter units designed for in vitro diagnostic use and intended for concentrating serum, urine, cerebrospinal fluid and other body fluids prior to analysis Learn More Amicon® Ultra Centrifugal Filters Fast and easy sample concentration Learn More Catalogue Number: UFC

Amicon® Ultra mL (10K NMWL) centrifugal filter was attached to the base of the exchange device. The resin was gently resuspended in 1 mL Elution Buffer (Ni-NTA Buffer Kit, Cat. No. ACRNB) and centrifuged at g for 15 min. In certain cases, the resulting sample OR Attach Amicon® Ultra device. Add elution buffer and resuspend resin. Amicon® Ultra K device — , NMWL Amicon® Ultra 10K filter devices are for in vitro diagnostic use and can be used to concentrate serum, urine, cerebrospinal fluid, and other body fluids prior to analysis. Amicon® Ultra 3K, 30K, 50K, and K filter devices are for research use only and not for use in diagnostic procedures. " Amicon® Ultra Centrifugal Filter Units are useful for protein purification and concentration. This filter device can remove proteins bellow 30 kDa. This filter device can be used to concentrate a pure protein with molecular mass above 30 kDa. The recommended velocity to spin this device is RPM, however when the filter is used for.

The Amicon® Ultra-4* and * Centrifugal Filter Units are the premier device for concentrating 1 – 4mL or of sample. These units are designed for in vitro diagnostic use and intended for concentrating serum, urine, cerebrospinal fluid and other body fluids prior to analysis. Amicon® Ultra Centrifugal Filter Unit Ultracel regenerated cellulose membrane, 15 mL sample volume; find Millipore-UFC MSDS, related peer-reviewed papers, technical documents, similar products more at Sigma-Aldrich. How to Use Amicon® Ultra Centrifugal Filter Devices 1. Add up to 15 mL of sample (12 mL if using a fixed-angle rotor) to the Amicon® Ultra filter device. 2. Place capped filter device into centrifuge rotor; counterbalance with a similar device. 3. When using a swinging-bucket rotor, s 15–60 minutes.